LOCUS       sequence-167614-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 transposon with a puromycin-thymidine kinase fusion driven by a
            PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pKTol2P-PTK
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    join(110..121,122..309)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(331..350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(332..350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     758..777
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      839..848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt
       61 actgtttatg taagcagaca gttttattgt tcatgatgca gctggatccc agaggtgtaa
      121 agtacttgag taattttact tgattactgt acttaagtat tatttttggg gatttttact
      181 ttacttgagt acaattaaaa atcaatactt ttacttttac ttaattacat ttttttagaa
      241 aaaaaagtac tttttactcc ttacaatttt atttacagtc aaaaagtact tattttttgg
      301 agatcacttc attctatttt cccttggtct ccctatagtg agtcgtatta gatcccggga
      361 ttactagttt aaacctagga tgcataccgg tcaagctagc gatctgagat ctggcctcgc
      421 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
      481 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
      541 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
      601 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      661 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
      721 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
      781 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaacgc
      841 caccatgggg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
      901 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
      961 cccggaccgc cacatcgagc gggtcaccga
//
