LOCUS       sequence-167616-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 transposon with a puromycin-thymidine kinase fusion driven by a
            PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pKTol2P-PTK
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(257..268,269..431)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(451..469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    570..651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    775..856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaacaga
      241 tttaaatggt accgagcaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
      301 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
      361 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt
      421 aaaagcaaga aagaaaacta gagtggtctc cctttagtga gggttaattg atctgaatga
      481 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      541 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      661 gtggtttgtc caaactcatc aatgaccaca tttgtagagg ttttacttgc tttaaaaaac
      721 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      781 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      841 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatga attcgccctt
      901 tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt
      961 cttcccaatc ctcccccttg ctgtcctgcc cca
//
