LOCUS       sequence-16762-e        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4051
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             633..653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             675..695
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             717..737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             759..779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(822..850)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttaactg atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc
       61 tgaaatcact cacaacgatg gatacgctaa caacttggaa atgaaataag cttgcatgcc
      121 tgcaggcctt ggtcgactct agaggatccc cggggattgg ccaaaggacc caaaggtatg
      181 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgct tggctagcaa
      241 aaatgcataa ggttttgctg gcactgttct ttatctttct ggcaccagca tccgcactgg
      301 cagtctccga accggcctgc aggtcgattt tttgcaaatt acgagcgttg tagggggcgg
      361 agcgataggt cctataggtt ttggtatatc atcattcatt cattcattgg tacattcatt
      421 tacccacctt cctctttctg agcttctctg gagttctgtg cttccttttt cccttatctt
      481 tatactgtaa tttttaactt tcaggcattg attggatccc cgggattggc caaaggaccc
      541 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttgctt
      601 ggagggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag gtaccggcag
      661 aaaaaatgac tgctccaaag aagaagcgta aggtaccggc agaaaaaatg actgctccaa
      721 agaagaagcg taaggtaccg gcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac
      781 cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      841 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      901 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      961 gggtaagttt aaacatatat atactaacta accntgatta tttaantttc agccaacact
     1021 tgtcactact ttcngtatgg ngtncaangc tcccnagana cccanatcaa tgaaacggca
     1081 tgacttttca aaatgccntg cccaangtta gtacaggaaa aactantttt tcaaaaagac
     1141 ggnactacan accgtangtt naacgttcgg actactaacc tactattaan tttcngggcg
     1201 aancagttga gggaaccctg taaaaaccga tnaaggntga ttnaaaanga antttggaca
     1261 ttggaaacct tancccnagn ttcccgggna nncaaaagaa nnagggngta nngnttnnaa
     1321 cnnttttttt tcactaatcc antgaagggc ntgnnaatca aacctggggn nnttaggggc
     1381 cttcttaaaa aangntt
//
