LOCUS       sequence-167621-        9158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-guide Perturb-seq vector backbone; modified bovine U6 promoter; 
            original constant region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE,
            Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit 
            A, Parnas O, Regev A, Weissman JS
  TITLE     A Multiplexed Single-Cell CRISPR Screening Platform Enables 
            Systematic Dissection of the Unfolded Protein Response.
  JOURNAL   Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 
            10.1016/j.cell.2016.11.048.
  PUBMED    27984733
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11"; journalName: "Cell"; date: "2016-12-15- 
            15"; volume: "167"; issue: "7"; pages: "1867-1882.e21"
COMMENT     Sequence Label: pMJ114
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..75)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        247..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     781..801
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             844..1024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1071..1196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1695..1928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2113..2157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2455..2572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2631..2855
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2844..2861
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2905..3603)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(3616..3669)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3679..4275)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3759..3779)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4256..4275
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          4296..5234
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4319..4339)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5235..5446)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    complement(5958..5991)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6047..6635
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6100..6120)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6518..6529)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6638..6654)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6639..6655)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7164..7344
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7406..7994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7486..7505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8165..8956,8957..9025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8788..8807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9026..9130)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga ccatagagcc
     2641 caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
     2701 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
     2761 ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg
     2821 gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac
     2881 gggccctcta ggccctagga attcttaatt aagcttgtgc cccagtttgc tagggaggtc
     2941 gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt
     3001 gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg
     3061 tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga tggctcccgc ccacgagctt
     3121 cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa
     3181 ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc
     3241 tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt
     3301 cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga
     3361 ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag
     3421 gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat
     3481 tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg
     3541 atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc
     3601 gctggtggcg accggtgggc caggattctc ctcgacgtca ccgcatgtta gcagacttcc
     3661 tctgccctct cccgatccgg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     3721 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     3781 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     3841 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
     3901 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     3961 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     4021 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
     4081 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     4141 ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     4201 ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     4261 cttgtactcg gtcatggtgg gctagctcac gacacctgaa atggaagaaa aaaactttga
     4321 accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag
     4381 gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga
     4441 aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagcgcgag
     4501 aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct
     4561 gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc
     4621 cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct ttccgctcac
     4681 gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg
     4741 aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat tctcggtggc
     4801 cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg
     4861 cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt tacaagacta
     4921 tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc tagagactta
     4981 tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg gccccagcgc
     5041 ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca aggcctcgaa
     5101 ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg cagccaggtg
     5161 gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc gcgggaacca
     5221 cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg
     5281 actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg
     5341 acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc
     5401 cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggcacat
     5461 gcatggcggt aatacggtta tccacgcggc cgcctaatgg atcctagtac tcgagcatcg
     5521 tgatagtcag actggaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
     5581 ctagccttat ttaaacttgc tatgctgttt ccagcttagc tcttaaacgg gtggtgccca
     5641 tcctggtcca taaacatggc ctctcaagaa ctatataaag ctaagaatag aaaaaatttt
     5701 acagttatgg tgactaaact gtaacccttt tttaagtttc aaagtatata ataagttatg
     5761 accttttgta ccacacacat tatcatcata tagtttgatt agggctaagt ttaatagtct
     5821 ccataacctt attacatcat atatgcaaat gaagggaacc atgggaaatt ggccctaggc
     5881 ccgcccgacc tctgcgcgtc tgtctagtgg actgcgcggg cagacaagct cggtcacggt
     5941 taacgtcgag ggacctaata acttcgtata gcatacatta tacgaagtta tacatgttta
     6001 agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga
     6061 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6121 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6181 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6241 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6301 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6361 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6421 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6481 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6541 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6601 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     6661 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6721 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6781 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6841 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6901 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6961 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     7021 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     7081 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     7141 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7321 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     7381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7441 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9121 gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
