LOCUS       sequence-167625-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 transposon with EGFP driven by minimal-CAGs promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pKTol2C-EGFP
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    join(102..113,114..301)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(323..342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(324..342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        428..731
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg cagctggatc ccagaggtgt aaagtacttg
      121 agtaatttta cttgattact gtacttaagt attatttttg gggattttta ctttacttga
      181 gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag aaaaaaaagt
      241 actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt ggagatcact
      301 tcattctatt ttcccttggt ctccctatag tgagtcgtat tagatcccgg gattactagt
      361 ttaaacctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      421 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      481 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      541 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      601 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      661 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      721 ctattaccat gg
//
