LOCUS       sequence-167632-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Passport transposon with a puromycin-thymidine kinase fusion driven 
            by a PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pPTn2P-PTK
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(352..371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(353..371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     729..748
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttaatcgcg gcctcgacgt ttcccgttga atatggctca taacacccct tgtattactg
       61 tttatgtaag cagacagttt tattgttcat gatgcagctg gatccgatta cagtgccttg
      121 cataagtatt cacccccttt ggacttttct acattttgtc atgctataac cacagattaa
      181 aatttatttc atcgtgagtt tatgtaatgg accaacacaa aatagtgcat catttggaag
      241 tggggggaaa tattacatgg atttcacaat tatttacaaa taaaaatctg aaaagtgttg
      301 agtgcatatg tattcacccc ctttactgtg aaacccctaa caaagatggc tccctatagt
      361 gagtcgtatt agatctgaga tctggcctcg cacacattcc acatccaccg gtaggcgcca
      421 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
      481 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
      541 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
      601 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
      661 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
      721 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
      781 atctccgggc ctttcgacct gcagccaacg ccaccatggg gaccgagtac aagcccacgg
      841 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
      901 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
      961 agctgcaaga actcttcc
//
