LOCUS       sequence-167634-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Passport transposon with a puromycin-thymidine kinase fusion driven 
            by a PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pPTn2P-PTK
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(501..519)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    620..701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    825..906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaacaga
      241 tttaaatggt accgattaca gtgccttgca taagtattca ccccccttgg actttttccc
      301 attatgtact gttactaact ggaattcaaa tagacttaaa taaacttttt cccgtttgat
      361 caacaaaaca tgcatagtac tttggaggtg caaaataaat tttattgtga cacaaacaat
      421 aatgagaaca aaaaagttga catctgttgg gtgcataagt attcaccccc ctgtgtcaat
      481 acttggtaga acctggtctc cctttagtga gggttaattg atctgaatga ccacatttgt
      541 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      601 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      721 caaactcatc aatgaccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      781 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      901 cactgcattc tagttgtggt ttgtccaaac tcatcaatga attcgccctt tggttcttt
//
