LOCUS       sequence-167640-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pcDNA3 plasmid contains a new poly-linker and single C-terminal
            flag-tag sequence for expression in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Sanjabi S, Williams KJ, Saccani S, Zhou L, Hoffmann A, Ghosh G, 
            Gerondakis S, Natoli G, Smale ST
  TITLE     A c-Rel subdomain responsible for enhanced DNA-binding affinity and 
            selective gene activation.
  JOURNAL   Genes Dev. 2005 Sep 15. 19(18):2138-51.
  PUBMED    16166378
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2005 Sep 15. 19(18):2138-51."
COMMENT     Sequence Label: c-Flag pcDNA3
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(142..160)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(143..160)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(180..197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    186..410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      456..884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(543..562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     753..774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(893..913)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt atctagactc gagggtaccg gatccgacta caaggacgac
      121 gatgacaagt aagaattcta ttctatagtg tcacctaaat gctagagctc gctgatcagc
      181 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      241 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      301 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
      361 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
      421 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
      481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
      721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
      841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
      901 tgtcagttag ggtgtggaaa gtcc
//
