LOCUS       sequence-167647-        3927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GNA13 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GNA13_p.L374I
FEATURES             Location/Qualifiers
     source          1..3927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1327..1426)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1443..1462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1444..1462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1467..1483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1467..1483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1914..2705
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2798..3386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3287..3306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3540..3557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3716..3743)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3835..3921)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcggac ttcctgccgt cgcggtccgt gctgtccgtg tgcttccccg
      241 gctgcctgct gacgagtggc gaggccgagc agcaacgcaa gtccaaggag atcgacaaat
      301 gcctgtctcg ggaaaagacc tatgtgaagc ggctggtgaa gatcctgctg ctgggcgcgg
      361 gcgagagcgg caagtccacc ttcctgaagc agatgcggat catccacggg caggacttcg
      421 accagcgcgc gcgcgaggag ttccgcccca ccatctacag caacgtgatc aaaggtatga
      481 gggtgctggt tgatgctcga gagaagcttc atattccctg gggagacaac tcaaaccaac
      541 aacatggaga taagatgatg tcgtttgata cccgggcccc catggcagcc caaggaatgg
      601 tggaaacaag ggttttctta caatatcttc ctgctataag agcattatgg gcagacagcg
      661 gcatacagaa tgcctatgac cggcgtcgag aatttcaact gggtgaatct gtaaaatatt
      721 tcctggataa cttggataaa cttggagaac cagattatat tccatcacaa caagatattc
      781 tgcttgccag aagacccacc aaaggcatcc atgaatacga ctttgaaata aaaaatgttc
      841 ctttcaaaat ggttgatgta ggtggtcaga gatcagaaag gaaacgttgg tttgaatgtt
      901 tcgacagtgt gacatcaata cttttccttg tttcctcaag tgaatttgac caggtgctta
      961 tggaagatcg actgaccaat cgccttacag agtctctgaa catttttgaa acaatcgtca
     1021 ataaccgggt tttcagcaat gtctccataa ttctgttctt aaacaagaca gacttgcttg
     1081 aggagaaggt gcaaattgtg agcatcaaag actatttcct agaatttgaa ggggatcccc
     1141 actgcttaag agacgtccaa aaattcctgg tggaatgttt ccggaacaaa cgccgggacc
     1201 agcaacagaa gcccttatac caccacttca ccactgctat caacacggag aacatccgcc
     1261 ttgttttccg tgacgtgaag gatactattc tgcatgacaa cctcaagcag attatgctac
     1321 agtgattgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1381 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     1441 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     1501 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     1561 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1621 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1681 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1741 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1801 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1861 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     1921 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1981 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2041 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2101 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2161 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2221 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2281 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2341 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2401 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2461 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2521 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2581 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2641 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2701 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2761 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2821 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2881 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2941 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3001 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3061 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3121 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3181 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3241 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3301 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3361 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3421 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3481 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3541 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3601 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3661 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3721 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3781 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3841 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3901 ctgagccttt cgttttattt gatgcct
//
