LOCUS       sequence-16765-e        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4054
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             251..271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             293..313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             335..355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             377..397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(440..468)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttactga tcttctaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggtcgact ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      181 atactaacat aacatagaac attttcagga ggacccttgg agggtaccga gctcagaaaa
      241 aatgactgct ccaaagaaga agcgtaaggt accggcagaa aaaatgactg ctccaaagaa
      301 gaagcgtaag gtaccggcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggc
      361 agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg
      421 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      481 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      541 taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat
      601 actaactaac cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg
      661 tgttcaatgc ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc
      721 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      781 gacacgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc
      841 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      901 taaagaagat ggaaacattc ttggacacaa ttggaataca actataactc acncatgtat
      961 acntcatggc agacaacaaa agatggaatc caagtngtag tttaacntga tttactaact
     1021 actatctgat ttaatttccg aacttccaat tagacccacn ttgaaatgga gcgtcactac
     1081 nnacctntca caatactcca ttgggaggcc tgtctttncn nanacntnct gtcnccantt
     1141 gccttnaaaa tccacaaaaa aaaccctggn cctctgattg accccncgga tnncnggggg
     1201 ggactccaan ctccnaaatg ggtttacttc ncccncnncc cctttatttn aatccnngng
     1261 ncgnccnntn cnttgnggna aaaanggcnc ncccnattnn ncttccnnng aattnttnac
     1321 cccggcgana aaaaancncc cgntccnccc cctttnaacc ntttg
//
