LOCUS       sequence-167658-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the mitochondria (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pMTS_mScarlet-i_N1
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    128..193
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(194..889)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(469..488)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     738..756
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      886..895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag
      181 atctgagtcc ggacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggagc
      241 gttcgtactg ttccaccacg gtgtagtcct cgttgtggga ggtgatgtcc aacttgcggt
      301 cgacgttgta ggcgccgggc atctgcacgg gcttcttggc cttgtaggtg gtcttgaagt
      361 ccgccaggta gcggccgccg tccttcaggc gcagggccat cttaatgtcg cccttcagca
      421 cgccgtcctc ggggtacaac cgctcggtgg acgcttccca gcccattgtc ttcttctgca
      481 ttacggggcc gtcaggaggg aagttggtgc cgcggagctt caccttgtag atcagggtgc
      541 cgtcctccag ggaggtgtcc tgggtcacgg tcacggcgcc gccgtcctcg aagttcatca
      601 cgcgctccca cttgaagccc tcggggaagg actgcttata gtagtcgggg atgtcggcgg
      661 ggtgcttgat gaaggccctg gagccgtaca tgaactgagg ggacaggatg tcccaggaga
      721 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      781 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgtgca
      841 ccttgaaccg catgaactcc ttgatcactg cctcgccctt gctcaccatg gtggcgaccg
      901 gtggatcccc caacgaatgg atcttggcgc gcggcactgg gagccgccgg gcc
//
