LOCUS       sequence-167659-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Maji B, Moore CL, Zetsche B, Volz SE, Zhang F, Shoulders MD, 
            Choudhary A
  TITLE     Multidimensional chemical control of CRISPR-Cas9.
  JOURNAL   Nat Chem Biol. 2017 Jan;13(1):9-11. doi: 10.1038/nchembio.2224. Epub
            2016 Oct 31.
  PUBMED    27820801
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2224"; journalName: "Nat Chem Biol"; date: 
            "2017-01"; volume: "13"; issue: "1"; pages: "9-11"
COMMENT     Sequence Label: PP7 guide
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..161
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     rep_origin      453..836
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(540..559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aactgaaaaa cgatacaagg ctgttagaga gtaattggaa ttaatttgac tgtaaacaca
       61 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
      121 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
      181 ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt
      241 ttagagctag gccggagcag acgatatggc gtcgctccgg cctagcaagt taaaataagg
      301 ctagtccgtt atcaacttgg ccggagcaga cgatatggcg tcgctccggc caagtggcac
      361 cgagtcggtg ctttttttga attctgatgc ggtattttct ccttacgcat ctgtgcggta
      421 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
      481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
      541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      661 acttgatttt gggtgatggt tcacgtagtg ggccatcgcc ctgattagac ggtttttcgc
      721 cctttgacgt tggagtccac gtttctttaa tagtgggact cttgttccaa actggaacaa
      781 cattcaactc tatctcgggc tattctttga tttataatgg attttgccga tttcggtcta
      841 tggtttaaaa ttgagcggat taacaaaatt taacgcgaat t
//
