LOCUS       sequence-167660-        3797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PSG2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PSG2_p.H245Y
FEATURES             Location/Qualifiers
     source          1..3797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1197..1296)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1313..1332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1314..1332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1337..1353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1337..1353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1784..2575
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2668..3256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3157..3176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3410..3427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3586..3613)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3705..3791)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggggccc ctctcagccc ctccctgcac agagcacatc aaatggaagg
      241 ggctcctgct cacagcatca cttttaaact tctggaacct gcccaccact gcccaagtca
      301 cgattgaagc ccagccacca aaagtttccg aggggaagga tgttcttcta cttgtccaca
      361 atttgcccca gaatcttact ggctacatct ggtacaaagg gcaaatcagg gacctctacc
      421 attacattac atcatatgta gtagacggtc aaataattat atatgggcct gcatatagtg
      481 gacgagaaac agcatattcc aatgcatccc tgctgatcca gaatgtcacc cgggaggacg
      541 caggatccta caccttacac atcataaagc gaggtgatgg gactagagga gtaactggat
      601 atttcacctt caccttatac ctggagactc ccaagccctc catctccagc agcaacttaa
      661 accccaggga ggccatggaa actgtgatct taacctgtga tcctgagact ccggacacaa
      721 gctaccagtg gtggatgaat ggtcagagcc tccctatgac tcacaggttt cagctgtccg
      781 aaaccaacag gaccctcttt ctatttggtg tcacaaagta tactgcagga ccctatgaat
      841 gtgaaatacg gaactcaggg agtgccagcc gcagtgaccc agtcaccctg aatctcctcc
      901 atggtccaga cctccccaga atttaccctt catacaccaa ttaccgttca ggagataacc
      961 tctacttgtc ttgcttcgcg aactctaacc caccggcaca gtattcttgg acaattaatg
     1021 ggaagtttca gcaatcagga caaaatctgt ttatccccca aattactaca aagcatagcg
     1081 ggctctatgt ttgctctgtt cgtaactcag ccactggcga ggaaagctcc acgtcgttga
     1141 cagtcaaagt ctctgcttct acaagaatag gacttcttcc tctccttatc caacataccc
     1201 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1261 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1321 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1381 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     1441 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     1501 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     1561 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     1621 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     1681 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1741 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     1801 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1861 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1921 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1981 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2041 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2101 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2161 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2221 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2281 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2341 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2401 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2461 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     2521 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     2581 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2701 cttgcaaaca aaaaaaccac cgctaccagc ggtggttttt ttgccggatc aagagctacc
     2761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3421 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3481 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3541 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
     3601 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     3661 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     3721 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
     3781 cgttttattt gatgcct
//
