LOCUS       sequence-167665-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S15A
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(881..904)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(938..989)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(967..987)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttgtcttccc aacttgcccc ttgctccata ccacccccct ccaccccata atattataga
       61 aggacaccta gtcagacaaa atgatgcaac ttaattttat taggacaagg ctggtgggca
      121 ctggagtggc aacttccagg gccaggagag gcactgggga ggggtcacag ggatgccacc
      181 cgggatccgg gctaaggctt tacttggcgg cagtctcatc ggattttgca gcttctgggc
      241 ccccaagctg ggcccgcgac tcgaaggtga ctgggatggt gatctcgttg gactgcgtgg
      301 ctagcttggg catgggggcc tccacggtca gtgtgccctc aggggacagg gaggaggaaa
      361 cttgggtggg gtccacaccg gggggcagcg tgtatttccg cgtgaagcac cgggagatgt
      421 agccatgctc gtcctgccgc tcctcgtgct tgccggtgat ctccaccacg ccatccttgg
      481 tcttgaccgt cagctcgtcc ggggcgaagt ggttgacatc cagggacacg cgccagcggt
      541 ccgcagtgtg ccggatctcc gagaccccgc tgctgagttg ccggctgagc gcgcggctgt
      601 aggcgggcgc ggccactgcg gggctctcga tggcggcggg gggcaggggg cgcacgtagc
      661 ctggccagct gctgccgcct aaccactgcg accactcctc cggcagccgg ggcagcccga
      721 aggcctggtc gaagaggcgg ctatgcgggt accagtcgcg gaaggggtcc caagcggggc
      781 cccgcaggag cgagaagggg acgcggcgct cggtcatgct ggatcctcta gagtcgactg
      841 gtaccgatat cagatctatc gatgaattcg cggccgcaag cttgtcgtca tcgtctttgt
      901 agtccatggt agatcaattc tgacggttca ctaaacgagc tctgcttata tagacctccc
      961 accgtacacg cctaccgccc atttgcgtca acggggcggg gttattacga cattttggaa
     1021 agtcccg
//
