LOCUS       sequence-167669-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the mitochondria (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pMTS_mScarlet-H_N1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    127..192
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(193..888)
                     /codon_start=1
                     /product="monomeric red fluorescent protein evolved from a 
                     synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-H
                     /note="more photostable than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     HALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(468..487)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     737..755
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      885..894
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt gagctcgaga
      181 tctgagtccg gacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggagcg
      241 ttcgtactgt tccaccacgg tgtagtcctc gttgtgggag gtgatgtcca acttgcggtc
      301 gacgttgtag gcgccgggca tctgcacggg cttcttggcc ttgtaggtgg tcttgaagtc
      361 cgccaggtag cggccgccgt ccttcaggcg cagggcgtgc ttaatgtcgc ccttcagcac
      421 gccgtcctcg gggtacaacc gctcggtgga cgcttcccag cccattgtct tcttctgcat
      481 tacggggccg tcaggaggga agttggtgcc gcggagcttc accttgtaga tcagggtgcc
      541 gtcctccagg gaggtgtcct gggtcacggt cacggcgccg ccgtcctcga agttcatcac
      601 gcgctcccac ttgaagccct cggggaagga ctgcttatag tagtcgggga tgtcggcggg
      661 gtgcttggtg aaggccctgg agccgtacat gaactgaggg gacaggatgt cccaggagaa
      721 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      781 gccctcgccc tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgtgcac
      841 cttgaaccgc atgaactcct tgatcactgc ctcgcccttg ctcaccatgg tggcgaccgg
      901 tggatccccc a
//
