LOCUS       sequence-167671-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E2-Crimson-FKBP(M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Barrero JJ, Papanikou E, Casler JC, Day KJ, Glick BS
  TITLE     An improved reversibly dimerizing mutant of the FK506-binding 
            protein FKBP.
  JOURNAL   Cell Logist. 2016 Jun 24;6(3):e1204848. eCollection 2016 Jul-Sep.
  PUBMED    27738551
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2016-06-24"; volume: "6(3)"; pages: "e1204848. 
            eCollection 2016 Jul-Sep"
COMMENT     Sequence Label: E2-Crimson-FKBP(M)
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             236..559
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     terminator      570..817
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(586..604)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(836..852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(836..852)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(849..871)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    860..876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(884..890,891..908,909..914))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    929..950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cccccgcgac ggcgtgctga agggcgagaa ccacatggcg ctgaagctga agggcggcgg
       61 ccactacctg tgtgagttca agtccatcta catggccaag aagcccgtga agctgcccgg
      121 ctaccactac gtggactaca agctcgacat cacctcccac aacgaggact acaccgtggt
      181 ggagcagtac gagcgcgccg aggcccgcca ccacctgttc cagggatcct ctagaggagt
      241 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
      301 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa atggattcct cccgggacag
      361 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
      421 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
      481 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
      541 gcttctaaaa ctggaataaa ctagagtcat catgtaatta gttatgtcac gcttacattc
      601 acgccctccc cccacatccg ctctaaccga aaagggagga gttagacaac ctgaagtcta
      661 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
      721 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
      781 tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag cttggcgtaa tcatggtcat
      841 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      901 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      961 gctcactgcc cgctttccag tcgggaaacc tgtcg
//
