LOCUS       sequence-167681-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E2-Crimson-FKBP(L,V) in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Barrero JJ, Papanikou E, Casler JC, Day KJ, Glick BS
  TITLE     An improved reversibly dimerizing mutant of the FK506-binding 
            protein FKBP.
  JOURNAL   Cell Logist. 2016 Jun 24;6(3):e1204848. eCollection 2016 Jul-Sep.
  PUBMED    27738551
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2016-06-24"; volume: "6(3)"; pages: "e1204848. 
            eCollection 2016 Jul-Sep"
COMMENT     Sequence Label: E2-Crimson-FKBP(L,V)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..324)
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             complement(1..324)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             complement(1..324)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(594..613)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttattccagt tttagaagct ccacatcgaa gacgagagtg gcatgtggtg ggataacgcc
       61 tgggtgccca gtggcaccat aggcataatc tggagatata gtcagtttgg ctctctgacc
      121 cacactcatc tgggcaaccc cttcttccca gcctcggatc acctcctgct tgcctagcat
      181 aaacttaaag ggcttgtttc tgtcccggga ggaatctagt ttctttccat cttcaagcat
      241 cccggtgtag tgcaccacgc aggtctggcc gcgcttgggg aaggtgcgcc cgtctcctgg
      301 ggagatggtt tccacctgca ctcctctaga ggatccctgg aacaggtggt ggcgggcctc
      361 ggcgcgctcg tactgctcca ccacggtgta gtcctcgttg tgggaggtga tgtcgagctt
      421 gtagtccacg tagtggtagc cgggcagctt cacgggcttc ttggccatgt agatggactt
      481 gaactcacac aggtagtggc cgccgccctt cagcttcagc gccatgtggt tctcgccctt
      541 cagcacgccg tcgcgggggt agttgcgctc agtggagggc tcccagccca gagtcttctt
      601 ctgcattacg gggccgtcgg aggggaagtt cacgccgatg aacttcacgt ggtagatgag
      661 ggtgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      721 catcacgcgc tcccacttga agccctcggg gaaggactgc ttgaggtagt cggggatgtc
      781 ggcggggtgc ttgatgtacg ccttggagcc gt
//
