LOCUS       sequence-167682-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E2-Crimson-FKBP(L,V) in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Barrero JJ, Papanikou E, Casler JC, Day KJ, Glick BS
  TITLE     An improved reversibly dimerizing mutant of the FK506-binding 
            protein FKBP.
  JOURNAL   Cell Logist. 2016 Jun 24;6(3):e1204848. eCollection 2016 Jul-Sep.
  PUBMED    27738551
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2016-06-24"; volume: "6(3)"; pages: "e1204848. 
            eCollection 2016 Jul-Sep"
COMMENT     Sequence Label: E2-Crimson-FKBP(L,V)
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             239..562
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             239..562
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             239..562
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     terminator      573..820
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(589..607)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(839..855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(839..855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(852..874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    863..879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(887..893,894..911,912..917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    932..953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctacccccgc gacggcgtgc tgaagggcga gaaccacatg gcgctgaagc tgaagggcgg
       61 cggccactac ctgtgtgagt tcaagtccat ctacatggcc aagaagcccg tgaagctgcc
      121 cggctaccac tacgtggact acaagctcga catcacctcc cacaacgagg actacaccgt
      181 ggtggagcag tacgagcgcg ccgaggcccg ccaccacctg ttccagggat cctctagagg
      241 agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac
      301 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaactagatt cctcccggga
      361 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga
      421 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc
      481 ctatggtgcc actgggcacc caggcgttat cccaccacat gccactctcg tcttcgatgt
      541 ggagcttcta aaactggaat aaactagagt catcatgtaa ttagttatgt cacgcttaca
      601 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
      661 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
      721 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
      781 gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagcttggcg taatcatggt
      841 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      961 tgcgctcact gcccgctttc cagtcgg
//
