LOCUS       sequence-167684-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid 
            vector with double cos sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Jones KM
  TITLE     Increased production of the exopolysaccharide succinoglycan enhances
            Sinorhizobium meliloti 1021 symbiosis with the host plant Medicago 
            truncatula.
  JOURNAL   J Bacteriol. 2012 Aug;194(16):4322-31. doi: 10.1128/JB.00751-12. 
            Epub 2012 Jun 8.
  PUBMED    22685282
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.00751-12"; journalName: "J Bacteriol"; date: "2012-08"; 
            volume: "194"; issue: "16"; pages: "4322-31"
COMMENT     Sequence Label: pstb-LAFR5
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     214..236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     228..245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     229..245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(728..750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    739..755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(763..769,770..787,788..793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    808..829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cctgggtggc ggcctgccct tcacttcggc cgtcggggca ttcacggact tcatggcggg
       61 gccggcaatt tttaccttgg gcattcttgg catagtggtc gcgggtgccg tgctcgtgtt
      121 cggggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      181 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      241 ccagtgccaa gcttggcacg cgtctgaatg tccattgttc cctcccgaaa tcgtgttccc
      301 cgagacagtc ctctctgtcc cggcgggagt atttctcaaa actcgggtag acgcaattat
      361 tttcagtaaa tttttacctc gtttttatga gcggcgacgg cgctttcggc cgtctagcct
      421 gcacaagcca tcttgctagc atcgcatgcg tctcctatat tgggggaaag gagtattgag
      481 gcatgggtgc cccacagctt tcggtccgaa gtgcaaaagc gcgtgatctg gcgcgccgac
      541 ttgcacggcg agagaaccgc tccatcgctg acatcgtcga gcgtgctctt gagttatatg
      601 aaaccagaga agctggccga gagcctgcga gtgctttcta tgcgcgcttg ttcgagagtt
      661 gcggcacgga catcgacctg caggtcgacg gatccccggg aattcgtaat catggtcata
      721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      901 acgcgcgggg agaggcggtt tgcgtatggg ttcgtgcgat ccgtctgcat gatggtgata
      961 gccgtcagca tgattttcgt gtcgtcgaac ttggtga
//
