LOCUS       sequence-167690-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S82A
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(967..986)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 taagcagagc tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg
       61 acgacaagct tgcggccgcg aattcatcga tagatctgat atcggtacca gtcgactcta
      121 gaggatccag catgaccgag cgccgcgtcc ccttctcgct cctgcggggc cccagctggg
      181 accccttccg cgactggtac ccgcatagcc gcctcttcga ccaggccttc gggctgcccc
      241 ggctgccgga ggagtggtcg cagtggttag gcggcagcag ctggccaggc tacgtgcgcc
      301 ccctgccccc cgccgccatc gagagccccg cagtggccgc gcccgcctac agccgcgcgc
      361 tcagccggca actcgccagc ggggtctcgg agatccggca cactgcggac cgctggcgcg
      421 tgtccctgga tgtcaaccac ttcgccccgg acgagctgac ggtcaagacc aaggatggcg
      481 tggtggagat caccggcaag cacgaggagc ggcaggacga gcatggctac atctcccggt
      541 gcttcacgcg gaaatacacg ctgccccccg gtgtggaccc cacccaagtt tcctcctccc
      601 tgtcccctga gggcacactg accgtggagg cccccatgcc caagctagcc acgcagtcca
      661 acgagatcac catcccagtc accttcgagt cgcgggccca gcttgggggc ccagaagctg
      721 caaaatccga tgagactgcc gccaagtaaa gccttagccc ggatcccggg tggcatccct
      781 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
      841 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
      901 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
      961 gcggggtcta ttgggaacca agctggagtg cagtg
//
