LOCUS       sequence-167693-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S82A
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(869..892)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(926..1025)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(955..975)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cttgcccctt gctccatacc acccccctcc accccataat attatagaag gacacctagt
       61 cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact ggagtggcaa
      121 cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg ggatccgggc
      181 taaggcttta cttggcggca gtctcatcgg attttgcagc ttctgggccc ccaagctggg
      241 cccgcgactc gaaggtgact gggatggtga tctcgttgga ctgcgtggct agcttgggca
      301 tgggggcctc cacggtcagt gtgccctcag gggacaggga ggaggaaact tgggtggggt
      361 ccacaccggg gggcagcgtg tatttccgcg tgaagcaccg ggagatgtag ccatgctcgt
      421 cctgccgctc ctcgtgcttg ccggtgatct ccaccacgcc atccttggtc ttgaccgtca
      481 gctcgtccgg ggcgaagtgg ttgacatcca gggacacgcg ccagcggtcc gcagtgtgcc
      541 ggatctccga gaccccgctg gcgagttgcc ggctgagcgc gcggctgtag gcgggcgcgg
      601 ccactgcggg gctctcgatg gcggcggggg gcagggggcg cacgtagcct ggccagctgc
      661 tgccgcctaa ccactgcgac cactcctccg gcagccgggg cagcccgaag gcctggtcga
      721 agaggcggct atgcgggtac cagtcgcgga aggggtccca gctggggccc cgcaggagcg
      781 agaaggggac gcggcgctcg gtcatgctgg atcctctaga gtcgactggt accgatatca
      841 gatctatcga tgaattcgcg gccgcaagct tgtcgtcatc gtctttgtag tccatggtag
      901 atcaattctg acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc
      961 taccgcccat ttgcgtcaac ggggcggggt tattacgaca ttttggaaag tcccgttgat
     1021 tttggtgc
//
