LOCUS       sequence-167697-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E2-Crimson-FKBP(L).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Barrero JJ, Papanikou E, Casler JC, Day KJ, Glick BS
  TITLE     An improved reversibly dimerizing mutant of the FK506-binding 
            protein FKBP.
  JOURNAL   Cell Logist. 2016 Jun 24;6(3):e1204848. eCollection 2016 Jul-Sep.
  PUBMED    27738551
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2016-06-24"; volume: "6(3)"; pages: "e1204848. 
            eCollection 2016 Jul-Sep"
COMMENT     Sequence Label: E2-Crimson-FKBP(L)
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             241..564
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             241..564
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             241..564
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     terminator      575..822
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(591..609)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(841..857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(841..857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(854..876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    865..881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(889..895,896..913,914..919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    934..955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcactccccc gcgacggcgt gctgaagggc gagaaccaca tggcgctgaa gctgaagggc
       61 ggcggccact acctgtgtga gttcaagtcc atctacatgg ccaagaagcc cgtgaagctg
      121 cccggctacc actacgtgga ctacaagctc gacatcacct cccacaacga ggactacacc
      181 gtggtggagc agtacgagcg cgccgaggcc cgccaccacc tgttccaggg atcctctaga
      241 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      301 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaactaga ttcctcccgg
      361 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      421 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      481 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      541 gtggagcttc taaaactgga ataaactaga gtcatcatgt aattagttat gtcacgctta
      601 cattcacgcc ctccccccac atccgctcta accgaaaagg gaggagttag acaacctgaa
      661 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
      721 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
      781 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagcttgg cgtaatcatg
      841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1021 cggcca
//
