LOCUS       sequence-167698-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the ER (can be used for colocalization 
            studies and the OSER assay).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pCytERM_mScarlet-H_N1
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      211..220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(350..368)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     618..637
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctggtac cccttgcagc ttccagttgt
       61 caggaagaga aggctcccat ggatcctgtg gtggtgctgg ggctctgtct ctcctgtttg
      121 cttctccttt cactctggaa acagagctat gggggaggga agcttcgaat tctgcagtcg
      181 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggcagtg
      241 atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc
      301 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      361 gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac
      421 ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa gcagtccttc
      481 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg
      541 acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc
      601 aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc
      661 gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagcacgc cctgcgcctg
      721 aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcc
//
