LOCUS       sequence-167699-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-FKBP(M)x4 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Barrero JJ, Papanikou E, Casler JC, Day KJ, Glick BS
  TITLE     An improved reversibly dimerizing mutant of the FK506-binding 
            protein FKBP.
  JOURNAL   Cell Logist. 2016 Jun 24;6(3):e1204848. eCollection 2016 Jul-Sep.
  PUBMED    27738551
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2016-06-24"; volume: "6(3)"; pages: "e1204848. 
            eCollection 2016 Jul-Sep"
COMMENT     Sequence Label: EGFP-FKBP(M)x4
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..326)
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(333..653)
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 agttattcca gttttagaag ctccacatcg aagacgagag tggcatgtgg tgggatgatg
       61 cctgggtgcc cagtggcacc ataggcataa tctggagata tagtcagttt ggctctctga
      121 cccacactca tctgggcaac cccttcttcc cagcctcgga tcacctcctg cttgcctagc
      181 ataaacttaa agggcttgtt tctgtcccgg gaggaatcca ttttctttcc atcttcaagc
      241 atcccggtgt agtgcaccac gcaggtctgg ccgcgcttgg ggaaggtgcg cccgtctcct
      301 ggggagatgg tttccacctg cactcctcta gtttccagtt ttagaagctc cacatcgaag
      361 acgagagtgg catgtggtgg gatgatgcct gggtgcccag tggcaccata ggcataatct
      421 ggagatatag tcagtttggc tctctgaccc acactcatct gggcaacccc ttcttcccag
      481 cctcggatca cctcctgctt gcctagcata aacttaaagg gcttgtttct gtcccgggag
      541 gaatccattt tctttccatc ttcaagcatc ccggtgtagt gcaccacgca ggtctggccg
      601 cgcttgggga aggtgcgccc gtctcctggg gagatggttt ccacctgcac tcctctagtt
      661 tccagttt
//
