LOCUS       sequence-167702-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector expressing GFP alongside Cas9 and 
            an sgRNA cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Walter DM, Venancio OS, Buza EL, Tobias JW, Deshpande C, Gudiel AA, 
            Kim-Kiselak C, Cicchini M, Yates TJ, Feldser DM
  TITLE     Systematic in vivo inactivation of chromatin regulating enzymes 
            identifies Setd2 as a potent tumor suppressor in lung 
            adenocarcinoma.
  JOURNAL   Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 
            10.1158/0008-5472.CAN-16-2159.
  PUBMED    28202515
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2017 Feb 15. pii: canres.2159.2016. doi: 
            10.1158/0008-5472.CAN-16-2159."
COMMENT     Sequence Label: LentiCRISPRv2GFP
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        77..325
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     77..97
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     248..267
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        342..417
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        479..690
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      709..718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
       61 ggttaattaa ggtaccgagg gcctatttcc catgattcct tcatatttgc atatacgata
      121 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
      181 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      241 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      301 tatcttgtgg aaaggacgaa acaccggaga cggacgtctc tgttttagag ctagaaatag
      361 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      421 tttgaattcg ctagctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
      481 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
      541 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
      601 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
      661 tttcgcaacg ggtttgccgc cagaacacag gaccggttct agagcgctgc caccatggac
      721 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
      781 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
      841 atcaagaaga acctgatcgg agcc
//
