LOCUS       sequence-167708-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-FKBP(M)x4 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Barrero JJ, Papanikou E, Casler JC, Day KJ, Glick BS
  TITLE     An improved reversibly dimerizing mutant of the FK506-binding 
            protein FKBP.
  JOURNAL   Cell Logist. 2016 Jun 24;6(3):e1204848. eCollection 2016 Jul-Sep.
  PUBMED    27738551
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2016-06-24"; volume: "6(3)"; pages: "e1204848. 
            eCollection 2016 Jul-Sep"
COMMENT     Sequence Label: EGFP-FKBP(M)x4
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     154..175
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             230..550
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             557..877
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
       61 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      121 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      181 cgccgggatc actctcggca tggacgagct gtacaaggga tcctctagag gagtgcaggt
      241 ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt
      301 gcactacacc gggatgcttg aagatggaaa gaaaatggat tcctcccggg acagaaacaa
      361 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc
      421 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc
      481 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct
      541 aaaactggaa actagaggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt
      601 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa
      661 aatggattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
      721 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac
      781 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
      841 cactctcgtc ttcgatgtgg agcttctaaa actggaaact agaggagtgc aggtg
//
