LOCUS       sequence-167720-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Isopentenol production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Kang A, George KW, Wang G, Baidoo E, Keasling JD, Lee TS
  TITLE     Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for 
            isopentenol production.
  JOURNAL   Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub
            2015 Dec 17.
  PUBMED    26708516
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-03"; volume: "34:25-35. doi"; pages: " 
            10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17"
COMMENT     Sequence Label: JBEI-12050
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(65..70,71..88,89..95)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    103..119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(854..865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgtt taggcacccc
       61 aggctttaca ctttatgctt ccggctcgta taatgtgtgg aattgtgagc ggataacaat
      121 ttcagaattc aaaagatctt aggaggaata taaaatgaaa aattgtgtca tcgtcagtgc
      181 ggtacgtact gctatcggta gttttaacgg ttcactcgct tccaccagcg ccatcgacct
      241 gggggcgaca gtaattaaag ccgccattga acgtgcaaaa atcgattcac aacacgttga
      301 tgaagtgatt atgggtaacg tgttacaagc cgggctgggg caaaatccgg cgcgtcaggc
      361 actgttaaaa agcgggctgg cagaaacggt gtgcggattc acggtcaata aagtatgtgg
      421 ttcgggtctt aaaagtgtgg cgcttgccgc ccaggccatt caggcaggtc aggcgcagag
      481 cattgtggcg gggggtatgg aaaatatgag tttagccccc tacttactcg atgcaaaagc
      541 acgctctggt tatcgtcttg gagacggaca ggtttatgac gtaatcctgc gcgatggcct
      601 gatgtgcgcc acccatggtt atcatatggg gattaccgcc gaaaacgtgg ctaaagagta
      661 cggaattacc cgtgaaatgc aggatgaact ggcgctacat tcacagcgta aagcggcagc
      721 cgcaattgag tccggtgctt ttacagccga aatcgtcccg gtaaatgttg tcactcgaaa
      781 gaaaaccttc gtcttcagtc aagacgagtt cccgaaagcg aactcaacgg ctgaagcgtt
      841 aggtgcattg cgcccggcct tcgataaagc aggaacagtc accgctggga acgcgtctgg
      901 tattaacgac ggtgctgccg
//
