LOCUS       sequence-167721-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S78A/S82A
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(965..984)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat tgatctacca tggactacaa agacgatgac
       61 gacaagcttg cggccgcgaa ttcatcgata gatctgatat cggtaccagt cgactctaga
      121 ggatccagca tgaccgagcg ccgcgtcccc ttctcgctcc tgcggggccc cagctgggac
      181 cccttccgcg actggtaccc gcatagccgc ctcttcgacc aggccttcgg gctgccccgg
      241 ctgccggagg agtggtcgca gtggttaggc ggcagcagct ggccaggcta cgtgcgcccc
      301 ctgccccccg ccgccatcga gagccccgca gtggccgcgc ccgcctacag ccgcgcgctc
      361 gctcggcaac tcgctagcgg ggtctcggag atccggcaca ctgcggaccg ctggcgcgtg
      421 tccctggatg tcaaccactt cgccccggac gagctgacgg tcaagaccaa ggatggcgtg
      481 gtggagatca ccggcaagca cgaggagcgg caggacgagc atggctacat ctcccggtgc
      541 ttcacgcgga aatacacgct gccccccggt gtggacccca cccaagtttc ctcctccctg
      601 tcccctgagg gcacactgac cgtggaggcc cccatgccca agctagccac gcagtccaac
      661 gagatcacca tcccagtcac cttcgagtcg cgggcccagc ttgggggccc agaagctgca
      721 aaatccgatg agactgccgc caagtaaagc cttagcccgg atcccgggtg gcatccctgt
      781 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
      841 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
      901 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
      961 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatc
//
