LOCUS       sequence-167722-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of NudB (E. coli) and PMD (S. cerevisiae) are under 
            control of Trc promoter for isopentenol production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD,
            Petzold CJ, Keasling JD, Lee TS
  TITLE     Metabolic engineering for the high-yield production of 
            isoprenoid-based C(5) alcohols in E. coli.
  JOURNAL   Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128.
  PUBMED    26052683
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-8"; volume: "5:11128. doi"; pages: " 
            10.1038/srep11128"
COMMENT     Sequence Label: JBEI-6833
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             735..746
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtataagcgt cccgtttcga tcttagtggt catctacgca caagatacga aacgggtgct
       61 gatgttgcag cggcgtgacg atcccgattt ctggcagtcg gtaaccggca gcgtggaaga
      121 gggtgaaacc gcgccgcaag ctgccatgcg cgaagtaaag gaagaggtca ccattgatgt
      181 tgtcgctgaa caactgacct taattgactg tcagcgcacg gtagagtttg aaattttttc
      241 acatttacgt catcgctatg cgccgggcgt gacgcgtaat acggaatcat ggttctgtct
      301 tgcgcttccg cacgagcggc agatcgtttt cactgaacat ctggcttaca agtggcttga
      361 tgcgcctgct gcggcggcgc tcactaagtc ctggagcaac cggcaggcga ttgaacagtt
      421 tgtaattaac gctgcctgag aattcggatc taggaggatt atgagatgac cgtttacaca
      481 gcatccgtta ccgcacccgt caacatcgca acccttaagt attgggggaa aagggacacg
      541 aagttgaatc tgcccaccaa ttcgtccata tcagtgactt tatcgcaaga tgacctcaga
      601 acgttgacct ctgcggctac tgcacctgag tttgaacgcg acactttgtg gttaaatgga
      661 gaaccacaca gcatcgacaa tgaaagaact caaaattgtc tgcgcgacct acgccaatta
      721 agaaaggaaa tggaatcgaa ggacgcctca ttgcccacat tatctcaatg gaaactccac
      781 attgtctccg aaaataactt tcctacagca gctggtttag cttcctccgc tgctggcttt
      841 gctgcattgg tctctgcaat tgctaagtta taccaattac cacagtcaac ttcagaaata
      901 tctagaatag caagaaaggg gtctggttca gcttgtagat cgttgtttgg cggatacgtg
      961 gcctgggaaa tgggaaaagc tgaagatggt catgattcca tgg
//
