LOCUS       sequence-167730-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 
            promoter for isopentenol production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD,
            Petzold CJ, Keasling JD, Lee TS
  TITLE     Metabolic engineering for the high-yield production of 
            isoprenoid-based C(5) alcohols in E. coli.
  JOURNAL   Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128.
  PUBMED    26052683
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-8"; volume: "5:11128. doi"; pages: " 
            10.1038/srep11128"
COMMENT     Sequence Label: JBEI-6822
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(58..63,64..81,82..88)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(847..858)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gtttaggcac cccaggcttt
       61 acactttatg cttccggctc gtataatgtg tggaattgtg agcggataac aatttcagaa
      121 ttcaaaagat cttaggagga atataaaatg aaaaattgtg tcatcgtcag tgcggtacgt
      181 actgctatcg gtagttttaa cggttcactc gcttccacca gcgccatcga cctgggggcg
      241 acagtaatta aagccgccat tgaacgtgca aaaatcgatt cacaacacgt tgatgaagtg
      301 attatgggta acgtgttaca agccgggctg gggcaaaatc cggcgcgtca ggcactgtta
      361 aaaagcgggc tggcagaaac ggtgtgcgga ttcacggtca ataaagtatg tggttcgggt
      421 cttaaaagtg tggcgcttgc cgcccaggcc attcaggcag gtcaggcgca gagcattgtg
      481 gcggggggta tggaaaatat gagtttagcc ccctacttac tcgatgcaaa agcacgctct
      541 ggttatcgtc ttggagacgg acaggtttat gacgtaatcc tgcgcgatgg cctgatgtgc
      601 gccacccatg gttatcatat ggggattacc gccgaaaacg tggctaaaga gtacggaatt
      661 acccgtgaaa tgcaggatga actggcgcta cattcacagc gtaaagcggc agccgcaatt
      721 gagtccggtg cttttacagc cgaaatcgtc ccggtaaatg ttgtcactcg aaagaaaacc
      781 ttcgtcttca gtcaagacga gttcccgaaa gcgaactcaa cggctgaagc gttaggtgca
      841 ttgcgcccgg ccttcgataa agcaggaaca gtcaccgctg ggaacgcgtc tggtattaac
      901 gac
//
