LOCUS       sequence-167731-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp27 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-CMV2-Hsp27-S15A/S78A/S82A
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(877..900)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(934..985)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(963..983)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cttcccaact tgccccttgc tccataccac ccccctccac cccataatat tatagaagga
       61 cacctagtca gacaaaatga tgcaacttaa ttttattagg acaaggctgg tgggcactgg
      121 agtggcaact tccagggcca ggagaggcac tggggagggg tcacagggat gccacccggg
      181 atccgggcta aggctttact tggcggcagt ctcatcggat tttgcagctt ctgggccccc
      241 aagctgggcc cgcgactcga aggtgactgg gatggtgatc tcgttggact gcgtggctag
      301 cttgggcatg ggggcctcca cggtcagtgt gccctcaggg gacagggagg aggaaacttg
      361 ggtggggtcc acaccggggg gcagcgtgta tttccgcgtg aagcaccggg agatgtagcc
      421 atgctcgtcc tgccgctcct cgtgcttgcc ggtgatctcc accacgccat ccttggtctt
      481 gaccgtcagc tcgtccgggg cgaagtggtt gacatccagg gacacgcgcc agcggtccgc
      541 agtgtgccgg atctccgaga ccccgctcgc gagttgccgc gcgagcgcgc ggctgtaggc
      601 gggcgcggcc actgcggggc tctcgatggc ggcggggggc agggggcgca cgtagcctgg
      661 ccagctgctg ccgcctaacc actgcgacca ctcctccggc agccggggca gcccgaaggc
      721 ctggtcgaag aggcggctat gcgggtacca gtcgcggaag gggtcccacg cggggccccg
      781 caggagcgag aaggggacgc ggcgctcggt catgctggat cctctagagt cgactggtac
      841 cgatatcaga tctatcgatg aattcgcggc cgcaagcttg tcgtcatcgt ctttgtagtc
      901 catggtagat caattctgac ggttcactaa acgagctctg cttatataga cctcccaccg
      961 tacacgccta ccgcccattt gcgtcaacgg ggc
//
