LOCUS       sequence-167733-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty sgRNA expression vector. sgRNA sequence can be cloned into 
            its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     Allele-specific locus binding and genome editing by CRISPR at the 
            p16INK4a locus.
  JOURNAL   Sci Rep. 2016 Jul 28;6:30485. doi: 10.1038/srep30485.
  PUBMED    27465215
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-28"; volume: "6:30485. doi"; pages: " 
            10.1038/srep30485"
COMMENT     Sequence Label: gRNA Cloning Vector Bbs I ver. 2
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(162..186)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(338..355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(448..1035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(528..547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgggt cttcgagaag
       61 acctgtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat
      121 caacttgaaa aagtggcacc gagtcggtgc tttttttcta gacccagctt tcttgtacaa
      181 agttggcatt agaattcaag cttattaatt aatggagcac aagactggcc tcatgggcct
      241 tccgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc
      301 atagctgttt ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct
      361 cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa
      421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1021 aaaaaaagga tctca
//
