LOCUS       sequence-167734-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKS/CS
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(164..250)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(330..348)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        414..516
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(583..602)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct
       61 ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca
      121 gtgagcgagg aagcggaata tatccctagg tctagggcgg cggatttgtc ctactcagga
      181 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
      241 gttttatttg atgccttggc ttcagggatg aggcgccatc attaccctgt tatccctatc
      301 accgtttgga ccttggggcc gtagaaaggg cctcgtgata cgcctatttt tataggttaa
      361 tgtcatgata ataatggttt cttagacgtc gatatctggc gaaaatgaga cgttgatcgg
      421 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
      481 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      541 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      601 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      661 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      721 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      781 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      841 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
      901 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
      961 cctgg
//
