LOCUS       sequence-167735-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty sgRNA expression vector. sgRNA sequence can be cloned into 
            its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     Allele-specific locus binding and genome editing by CRISPR at the 
            p16INK4a locus.
  JOURNAL   Sci Rep. 2016 Jul 28;6:30485. doi: 10.1038/srep30485.
  PUBMED    27465215
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-28"; volume: "6:30485. doi"; pages: " 
            10.1038/srep30485"
COMMENT     Sequence Label: gRNA Cloning Vector Bbs I ver. 2
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(63..311)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(121..140)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(291..311)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(441..460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(442..460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(471..488)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(471..487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(480..502)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        803..907
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccttatttaa acttgctatg ctgtttccag catagctctt aaacaggtct tctcgaagac
       61 ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
      121 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
      181 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
      241 attccaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
      301 aataggccct cttcctgccc gaccttggta ccggatccag tcgactgaat tggttccttt
      361 aaagcctgct tttttgtaca gaattctcga gtggcgcgcc tggacaagac acgtggcctt
      421 gacggccttc cgccaattcg ccctatagtg agtcgtatta cgtcgcgctc actggccgtc
      481 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      541 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgaaac gcccttccca
      601 acagttgcgc agcctgaatg gcgaatggga gcgccctgta gcggccactc aaccctatct
      661 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      721 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
      781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttga
//
