LOCUS       sequence-167740-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp40/DnaJB1 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Flag-Hsp40-S149A/S151A
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             133..156
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggt accgagctcg gatccaagag agataccacc
      121 atggccctcg aggactacaa ggacgacgat gacaagtcta gaggtaaaga ctactaccag
      181 acgttgggcc tggcccgcgg cgcgtcggac gaggagatca agcgggccta ccgccgccag
      241 gcgctgcgct accacccgga caagaacaag gagcccggcg ccgaggagaa gttcaaggag
      301 atcgctgagg cctacgacgt gctcagcgac ccgcgcaagc gcgagatctt cgaccgctac
      361 ggggaggaag gcctaaaggg gagtggcccc agtggcggta gcggcggtgg tgccaatggt
      421 acctctttca gctacacatt ccatggagac cctcatgcca tgtttgctga gttcttcggt
      481 ggcagaaatc cctttgacac cttttttggg cagcggaacg gggaggaagg catggacatt
      541 gatgacccat tctctggctt ccctatgggc atgggtggct tcaccaacgt gaactttggc
      601 cgcgcccgcg ctgcccaaga gcccgcccga aagaagcaag atcccccagt cacccacgac
      661 cttcgagtct cccttgaaga gatctacagc ggctgtacca agaagatgaa aatctcccac
      721 aagcggctaa accccgacgg aaagagcatt cgaaacgaag acaaaatatt gaccatcgaa
      781 gtgaagaagg ggtggaaaga aggaaccaaa atcactttcc ccaaggaagg agaccagacc
      841 tccaacaaca ttccagctga tatcgtcttt gttttaaagg acaagcccca caatatcttt
      901 aagagagatg gctctgatgt catttatcct gccaggatca gcctccggga ggctctgtgt
      961 ggctgcacag tgaacgtccc cactctggac ggca
//
