LOCUS       sequence-167741-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow/red FRET biosensor for monotoring plasmamembrane 
            PtdIns(4,5)P2 levels (PIP2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(648..899)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(690..711)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      121 ctagatccgg tggatcctca gatgttgagc tccttcagga agttctgcag ctccttgaag
      181 ctcatcttgt tgtccttgtt tttgtcagct tttcgcaagc aggagtgaat ccagtgctgt
      241 agcttctgac gctggtccat ggagcctgag tggtggatga tcttgtgcag ccccagcacc
      301 cagtgctggg catcagctgg cgatggggcg atgaggtcta gtgtattgcg ctggtccttg
      361 aagacaatgg agaagcagcg gtcctcgggc acatcacggg cgaacttctc cagaccctcc
      421 gtgcggtgcc ccattcgcac ctcctgaatg tcctcgatgg agaacagctg ggactccggg
      481 gtccgcatga ccttgcggga ctcctgccag atggtcttgc agtcctcctg caacttgtag
      541 aagcgctctc tcctccatga gctggacttc accttcagga gctggctgcc cttcagcagc
      601 gcctgtagat cctcatcatc ctgtaggcct cgagatctga gtccggactt gtacagctcg
      661 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      721 ttctcgttgg ggtctttgct cagcttggac tggtagctca ggtagtggtt gtcgggcagc
      781 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg
      841 ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg
      901 gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      961 ccgtcctcct tgaagtcgat g
//
