LOCUS       sequence-167744-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hsp40/DnaJB1 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  TITLE     Moens lab plasmids
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Flag-Hsp40-S171A
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             131..154
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccaagagag ataccaccat
      121 ggccctcgag gactacaagg acgacgatga caagtctaga ggtaaagact actaccagac
      181 gttgggcctg gcccgcggcg cgtcggacga ggagatcaag cgggcctacc gccgccaggc
      241 gctgcgctac cacccggaca agaacaagga gcccggcgcc gaggagaagt tcaaggagat
      301 cgctgaggcc tacgacgtgc tcagcgaccc gcgcaagcgc gagatcttcg accgctacgg
      361 ggaggaaggc ctaaagggga gtggccccag tggcggtagc ggcggtggtg ccaatggtac
      421 ctctttcagc tacacattcc atggagaccc tcatgccatg tttgctgagt tcttcggtgg
      481 cagaaatccc tttgacacct tttttgggca gcggaacggg gaggaaggca tggacattga
      541 tgacccattc tctggcttcc ctatgggcat gggtggcttc accaacgtga actttggccg
      601 ctcccgctct gcccaagagc ccgcccgaaa gaagcaagat cccccagtca cccacgacct
      661 tcgagtcgcc cttgaagaga tctacagcgg ctgtaccaag aagatgaaaa tctcccacaa
      721 gcggctaaac cccgacggaa agagcattcg aaacgaagac aaaatattga ccatcgaagt
      781 gaagaagggg tggaaagaag gaaccaaaat cactttcccc aaggaaggag accagacctc
      841 caacaacatt ccagctgata tcgtctttgt tttaaaggac aagccccaca atatctttaa
      901 gagagatggc tctgatgtca tttatcctgc caggatcagc ctccgggagg ctctgtgtgg
      961 ctgcacagtg aacgtcccca ctctggacgg caggacgata cccgtcgtat tcaaagatgt
     1021 tatcaggcct ggcatgcggc gaaaagttcc tggagaaggc ctccccctcc ccaaaa
//
