LOCUS       sequence-167745-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian vector for the expression of a dual read-out BRET-FRET 
            sensor for intracellular Zn2+ imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Aper SJ, Dierickx P, Merkx M
  TITLE     Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc.
  JOURNAL   ACS Chem Biol. 2016 Oct 21;11(10):2854-2864. Epub 2016 Sep 1.
  PUBMED    27547982
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2016-10-21"; volume: "11(10)"; pages: "2854-2864. 
            Epub 2016 Sep 1"
COMMENT     Sequence Label: pCMV-BLZinCh-1
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(507..509,510..512,513..1025)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(552..573)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(813..832)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtatttac tcttgaagat tttgtcggtg attggcgcca gaccgccggc tataacctgg
       61 accaagtgct tgaacagggc ggggttagca gcctgtttca aaacctgggg gtgagtgtca
      121 cgccaattca gcgcatcgtt ctgtcgggag agaatggtct gaaaatcgat atccacgtca
      181 ttatcccgta cgaaggtctt tctggtgatc agatggggca gatagaaaaa atattcaaag
      241 tggtgtaccc agtagacgat catcacttca aggttatact gcactatggc accctcgtta
      301 tcgatggcgt tactccgaat atgatcgatt actttgggcg tccttatgaa ggtattgcgg
      361 tgttcgacgg taaaaaaatt acggttaccg ggacgctctg gaatggtaat aaaatcattg
      421 atgagcgctt gataaaccca gatggcagcc ttctgttcag agttacgata aacggggtta
      481 cgggttggcg actgtgcgaa agacatatgg tgagcaaggg cgaggagctg ttcaccgggg
      541 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      601 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      661 gtaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct
      721 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      781 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      841 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      901 aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatcagc gacaacgtct
      961 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1021 tcgaggacgg cagcgtgc
//
