LOCUS       sequence-16776-e        1310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4119
FEATURES             Location/Qualifiers
     source          1..1310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     46..62
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(746..762)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(747..763)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(787..806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(816..833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(816..832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(825..847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctcctaagg gacaaaagct ggagctccac cgcggtggcg gccgctctag aactagtgga
       61 tccaccggtg ctccactatt agaggatacg gaatgctgtg gaaaagagag atgtcagcat
      121 gcgactgaag aatctgaata caagaaactt gtatatgtag ctgtgcatct tgtgaatgta
      181 ctcgaacgac tatctcttcc ttctgcacat atcgatgatt ccgatgagga aaaagagccg
      241 aaagccgacg ttcgaagact gatgatcgtg tgccatgaat tacatattcc acttctacat
      301 catacacttg aagagaggat tcctgatagt aacagtgcag agttgggact tcccaaagct
      361 gcagttttgg aacaacttct agaacttgac ctagatgtga atgccactga taagaataac
      421 gacactccaa tgcacattct gcttcgagcc agagagttta ggaaaagctt ggttcgagca
      481 ctgctcgtac gtggcacatg gctgttcgca aggaatcggc acggagatgt ggtgttgaat
      541 gtgatgaaac gtatgaaagc gttgaatcac gccaactttg atgatctacc actaggtgcg
      601 tgtcgaatca gaaattgaga gttattagat tatttggaac gcgaataact tcattatacc
      661 acggagagag ccagaaattg aagaaattgg ttaaggagaa tggaatttaa tgaataatag
      721 cgcaccaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc
      781 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
      841 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc cccctttcgc
      901 cagctggcgt aatagcgaag aggcccgcac cgatcgcccn ttcccaacag ttgcgcagcc
      961 tgaatggcga atgggacgcc nccctgtagc gggcgcatta agcgcggcgg ggngtgggng
     1021 gttacncncc agcgtgaccc nctacccttg ccagggcccc tancgcccgc tcctttcnct
     1081 ttncttcccn tnccttttcc nccncgttcg ncgggctttn cccnggccag ctnctaaatc
     1141 cgggggcccc ccttnagggt tcccaattna gngcttnacg ccccccccnc cccaaaaaac
     1201 tngatnaggg ggngggtccc ccnnaggggc ccncccccct aaaaaacgnt tttccccctt
     1261 ngacgttggg ncccccnttt ttnaaanggn acccttttnc cnaannggaa
//
