LOCUS       sequence-167764-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for dCas9 with Blasticidin selectable 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH125_dCas9-Blast
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..71
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tgtcgtgacg tacggccacc atgaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
       61 aaaagaaaaa ggacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct
      121 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
      181 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
      241 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
      301 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
      361 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
      421 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
      481 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
      541 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
      601 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
      661 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
      721 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
      781 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
      841 tggccgagga tgc
//
