LOCUS       sequence-167765-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for dCas9 with Blasticidin selectable 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH125_dCas9-Blast
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca
       61 gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg
      121 gcacgttgtc gctcttgccc cgggccttgt cgcttctggt cagcaccttg ttgtcgatgg
      181 agtcgtcctt cagaaagctc tgaggcacga tgtggtccac atcgtagtcg gacagccggt
      241 tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca
      301 gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca
      361 gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct
      421 gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca
      481 tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg
      541 ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg
      601 ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc
      661 tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc
      721 ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca
      781 gctgcttcat cactttgtcg tcga
//
