LOCUS       sequence-167767-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for wtGFP S65T-Q80H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH314_wtGFP S65T, Q80H
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(150..171)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     499..518
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     758..779
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattgt acctagtgga accggaaccc
       61 ttaaacatgt ctcgacctgg cttactagtc ggttcaactc tagattactt gtacagctcg
      121 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      181 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
      241 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      301 ccgtcctcga tgttgtggcg gatcttgaag ttcgccttga tgccgttctt ctgcttgtcg
      361 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      421 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      481 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      541 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt ggtgcttcat gtggtcgggg
      601 tagcggctga agcactgcac gccgtaggtg aaggtggtca cgagggtggg ccagggcacg
      661 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      721 tcgccctcgc cagacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      781 atgggcacca ccccggtga
//
