LOCUS       sequence-16777-e        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4129
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(138..154)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(139..155)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(824..843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(825..843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(853..870)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(853..869)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(862..884)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gnttacctcc taagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact
       61 agcgnatcca ccggttccat ggctagccac gtgacgcgtg gatcccccgg gctgcaggaa
      121 ttcgatatca agcttatcga taccgtcgac ctcgaggatg ccaagagacg ccaagcccaa
      181 aagatcaacg agctccaaga ggctcttgat gccgccaact ctaagaacgc ttctcttgag
      241 aagaccaagt ccagacttgt cggagacttg gacgacgctc aagttgatgt cgagagagcc
      301 aacggtgtcg ccagtgctct tgagaagaag caaaagggct tcgacaagat catcgacgaa
      361 tggagaaaga agaccgacga cttggccgct gagcttgatg gagctcaacg cgaccttcgc
      421 aacacctcca ctgatctctt caaggccaag aacgcccaag aggagctcgc cgaggttgtt
      481 gagggactcc gccgtgagaa caagagcttg agccaagaga tcaaggatct taccgatcaa
      541 ctcggagagg gaggacgctc tgtccacgaa atgcaaaaga tcatccgccg tcttgagatt
      601 gagaaggaag aactccaaca cgctttggac gaggctgagg ctgcccttga agctgaagag
      661 agcaaggttc tccgcgccca ggttgaagtt tcccagatcc gttccgaaat cgagaaacgc
      721 atccaggaga aggaggaaga gttcgagaac acgagaaaga accacgcccg cgctcttgaa
      781 tcaatgcaag cttccctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
      841 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      901 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaaaagg
      961 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     1021 gtagcggcgc attaagcgcg gngggtgtgg tggtnacncg cagcgngacn gctaccttgc
     1081 cagggccnta ncnccngctc cttnnctttc ttccttcctt tcccccccgt tcgccggttt
     1141 tccngtnagc tntaaatcgg ggnttccttt aggttcnnat taggntttac gncccccccc
     1201 ccaaaantng atnnggggtg gttcngtagg ggcccccccn tgaaanngtt ttcccnttng
     1261 gtggagcccn ntntttaagg gacttnttca aangaaaanc ccnccctttt ggnttttttt
     1321 tnttangaat tnncannccc cttgttnaan gggtttnaaa attcncnttt naaattnnt
//
