LOCUS       sequence-167774-        3801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CYLC2 ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CYLC2_p.E133K
FEATURES             Location/Qualifiers
     source          1..3801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1201..1300)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1317..1336)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1318..1336)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1341..1357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1341..1357)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1788..2579
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2672..3260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3161..3180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3414..3431
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3590..3617)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3709..3795)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtctctc ccaagattcc aaagagtaaa ctttgggcca tatgataatt
      241 acattccagt cagtgaatta agcaaaaaat catggaatca gcaacacttt gccctgttat
      301 ttcccaaacc acaacggcca ggaaccaaaa ggagatcaaa accttctcaa atacgggaca
      361 acacggtttc tataattgat gaagaacaat taagaggaga tcgtagacaa ccattatgga
      421 tgtaccgttc tttaatgaga atttctgaga gaccatctgt ttatttagct gccaggaggc
      481 agcctctcaa accaactcgt actgtcgagg tggattctaa agcagcagaa attggtaaga
      541 aaggtgaaga caagacaaca cagaaggaca caacagattc ggaatcaaaa ttaaaacaag
      601 gaaaaaaaga ttcaaagaaa ggcaaggata tagagaaagg aaaagaagaa aagctagatg
      661 caaagaaaga tagcaaaaaa ggtaaaaagg atgcagagaa gggcaaagac tcagcaacag
      721 aatctgaaga tgaaaaagga ggtgcaaaga aagataacaa aaaagataaa aaggattcaa
      781 acaaaggcaa agactcggca acagaatctg aaggtgaaaa aggaggtaca gagaaagata
      841 gcaaaaaagg taaaaaggat tcaaagaagg gcaaggattc agccatagaa ttacaagctg
      901 taaaagcaga tgaaaagaag gatgaggatg gaaaaaaaga tgcaaacaaa ggtgatgaat
      961 cgaaggatgc caagaaagat gcaaaggaga ttaaaaaagg taagaaagat aagaagaagc
     1021 ccagtagtac agacagtgac tcaaaggatg atgtcaagaa agagtctaag aaggacgcca
     1081 cgaaagatgc caagaaagtt gccaagaaag atactgagaa agaatctgct gattcaaaga
     1141 aggatgcaaa gaaaaatgct aagaaggatg caaagaagga tgcaaagaag aagggcaagt
     1201 tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     1261 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1321 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1381 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     1441 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     1501 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     1561 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1621 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     1681 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     1741 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     1801 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     1861 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     1921 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     1981 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2041 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2101 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2161 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2221 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2281 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2341 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2401 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2461 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     2521 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     2581 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     2641 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3001 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3061 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3301 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3361 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3421 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3481 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3541 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     3601 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     3661 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     3721 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     3781 ctttcgtttt atttgatgcc t
//
