LOCUS       sequence-167777-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HRH4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HRH4_p.S170L
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1366..1465)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1482..1501)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1483..1501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1506..1522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1506..1522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1953..2744
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2837..3425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3326..3345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3579..3596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3755..3782)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3874..3960)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccagat actaatagca caatcaattt atcactaagc actcgtgtta
      241 ctttagcatt ttttatgtcc ttagtagctt ttgctataat gctaggaaat gctttggtca
      301 ttttagcttt tgtggtggac aaaaacctta gacatcgaag tagttatttt tttcttaact
      361 tggccatctc tgacttcttt gtgggtgtga tctccattcc tttgtacatc cctcacacgc
      421 tgttcgaatg ggattttgga aaggaaatct gtgtattttg gctcactact gactatctgt
      481 tatgtacagc atctgtatat aacattgtcc tcatcagcta tgatcgatac ctgtcagtct
      541 caaatgctgt gtcttataga actcaacata ctggggtctt gaagattgtt actctgatgg
      601 tggccgtttg ggtgctggcc ttcttagtga atgggccaat gattctagtt tcagagtctt
      661 ggaaggatga aggtagtgaa tgtgaacctg gatttttttt ggaatggtac atccttgcca
      721 tcacatcatt cttggaattc gtgatcccag tcatcttagt cgcttatttc aacatgaata
      781 tttattggag cctgtggaag cgtgatcatc tcagtaggtg ccaaagccat cctggactga
      841 ctgctgtctc ttccaacatc tgtggacact cattcagagg tagactatct tcaaggagat
      901 ctctttctgc atcgacagaa gttcctgcat cctttcattc agagagacag aggagaaaga
      961 gtagtctcat gttttcctca agaaccaaga tgaatagcaa tacaattgct tccaaaatgg
     1021 gttccttctc ccaatcagat tctgtagctc ttcaccaaag ggaacatgtt gaactgctta
     1081 gagccaggag attagccaag tcactggcca ttctcttagg ggtttttgct gtttgctggg
     1141 ctccatattc tctgttcaca attgtccttt cattttattc ctcagcaaca ggtcctaaat
     1201 cagtttggta tagaattgca ttttggcttc agtggttcaa ttcctttgtc aatcctcttt
     1261 tgtatccatt gtgtcacaag cgctttcaaa aggctttctt gaaaatattt tgtataaaaa
     1321 agcaacctct accatcacaa cacagtcggt cagtatcttc ttgattgcca actttcttgt
     1381 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     1441 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1501 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1561 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     1621 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     1681 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     1741 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     1801 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1861 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1921 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     1981 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2041 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2101 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2161 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2221 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2281 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2341 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2401 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2461 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2521 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2581 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2641 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2701 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     2761 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3541 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3601 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3661 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3721 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3781 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3841 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3901 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3961 atgcct
//
