LOCUS       sequence-167782-        3744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NFKBIA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NFKBIA_p.P137S
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1144..1243)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1260..1279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1261..1279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1284..1300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1284..1300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1731..2522
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2615..3203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3104..3123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3357..3374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3533..3560)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3652..3738)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgttccag gcggccgagc gcccccagga gtgggccatg gagggccccc
      241 gcgacgggct gaagaaggag cggctactgg acgaccgcca cgacagcggc ctggactcca
      301 tgaaagacga ggagtacgag cagatggtca aggagctgca ggagatccgc ctcgagccgc
      361 aggaggtgcc gcgcggctcg gagccctgga agcagcagct caccgaggac ggggacccgt
      421 tcctgcactt ggccatcatc catgaagaaa aggcactgac catggaagtg atccgccagg
      481 tgaagggaga cctggccttc ctcaacttcc agaacaacct gcagcagact ccactccact
      541 tggctgtgat caccaaccag ccagaaattg ctgaggcact tctgggagct ggctgtgatt
      601 ctgagctccg agactttcga ggaaataccc ccctacacct tgcctgtgag cagggctgcc
      661 tggccagcgt gggagtcctg actcagtcct gcaccacccc gcacctccac tccatcctga
      721 aggctaccaa ctacaatggc cacacgtgtc tacacttagc ctctatccat ggctacctgg
      781 gcatcgtgga gcttttggtg tccttgggtg ctgatgtcaa tgctcaggag ccctgtaatg
      841 gccggactgc ccttcacctc gcagtggacc tgcaaaatcc tgacctggtg tcactcctgt
      901 tgaagtgtgg ggctgatgtc aacagagtta cctaccaggg ctattctccc taccagctca
      961 cctggggccg cccaagcacc cggatacagc agcagctggg ccagctgaca ctagaaaacc
     1021 ttcagatgct gccagagagt gaggatgagg agagctatga cacagagtca gagttcacgg
     1081 agttcacaga ggacgagctg ccctatgatg actgtgtgtt tggaggccag cgtctgacgt
     1141 tatgcccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1201 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1261 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1321 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     1381 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1441 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1501 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1561 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1621 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1681 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     1741 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     1801 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     1861 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     1921 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     1981 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2041 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2101 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2161 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2221 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2281 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2341 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2401 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2461 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2521 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2581 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2641 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2701 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2761 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2821 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2881 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2941 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3001 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3061 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3301 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3361 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3421 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3481 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3541 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3601 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3661 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     3721 agcctttcgt tttatttgat gcct
//
