LOCUS       sequence-16779-e        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4271
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..756
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(73..101)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     696..716
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatacctcct aaagggaaca aaagctggta ccggtagaaa aaatgagtaa aggagaagaa
       61 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      121 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      181 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttctcttat
      241 ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt tttcaagagt
      301 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      361 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      421 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      481 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      541 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      601 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      661 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      721 gctgggatta cncatggcat ggatgaacta tacaaatgcc cgggggatcc cctagttcta
      781 gagcggcccc ccccctcgag gtcgacngta tcgatnagct tgatatcgna ttccggaaga
      841 tgggctccca tgcnccanag ttnaacgatn ccgaangctc cnattgatgg tctccangaa
      901 nggacanacc ntatccnttc ccgtgtccaa ggccngccca ncanggaang anccttcnga
      961 ngccnttgtc tactggataa gngaccccaa agccaagaat cccatatgat gnccgnaaaa
     1021 acccnactcc cgnanctcnc ccaccnggga acgctgatcc tgtccccctt gaaggggaac
     1081 ccccaaacnc tgacgnaggn nccccccccc ccatancccc ctnaaaanan ggnccaccnn
     1141 gnaaaaaccg gcaccangcc cnagnnttcc ngnaannaaa ccatgccaan tccnggccnc
     1201 cngnnngaaa aaaccccttt ccgnggaagn tgttaanggc naccgnaang ctncancccn
     1261 ccnaagnttt gccancnnaa anntgncccg tttnnncnca nccnanaccn cnctcctgga
     1321 acanggtttn cntccccccn ccccccncta ggcgntcntc nnacnncccc atnnaanagg
     1381 aacnanannc tntgtcnncc tcangctttc ttttccncnn caaaccnngn gggcngnant
     1441 ncanccccgc ncnctnnaca tattcccctc
//
