LOCUS       sequence-167802-         555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: contains 5-state register that regulates expression 
            of GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR163
FEATURES             Location/Qualifiers
     source          1..555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(5..54)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    192..240
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 gaggctgttt cgtcctcacg gactcatcag accggaaagn acatccggtg acagctgtgc
       61 catccctcgt cctcacccgc acttgagtcc acggctgatg ccaacacaat taacatcaga
      121 atcaaggtaa atgctttttg ctttttttgc gggctcacta gggaatggng ncgtccgtga
      181 cnttctgtgc gcggccggct tgtcgacnac ggcggtctcc gtcgtcagga tcatccgggc
      241 cagcggctgt ctatgccaga antcggtcan gtcggagtcc tagaaaggag ttttttagtt
      301 accttaattc taataaacga aataaaaact cgccgttcgc gcgacatgac atgngncgcc
      361 gattgattca gaaagttaaa caaaattatt tgtagaggga aaccgttgtg gtctcccnga
      421 atatattata cnagccttat gcatgcccgt aaagttatcc agcaaccact cntagancta
      481 nggcagcaga tagggacgac gtggtgttag ctgtgacaga cctcccactc acactcccca
      541 ggcatcaaat aaaac
//
