LOCUS       sequence-167809-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing FGF-responsive regulatory element of the 
            zebrafish dusp6 promoter for FGF-responding cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: p5E-dusp6
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..190)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        191..209
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    222..329
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     246..262
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     246..262
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(296..312)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgcggccgcc ctgcagctct agagctcgaa ttctacaggt cactaatacc atctaagtag
       61 ttggttcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      121 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aacttttttg
      181 tacaaacttg aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
      241 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
      301 ccgtcgacct cgaggggggg cccggtaccg tgagacctta aaactgcgga gaagacgcgc
      361 tgcaagaggc cgtagacgtc tcaggcctgg agcattgagc tttttacacg ccaaagtctc
      421 ttgtgcgccg tcgagatgat taaaatcctc gttttttgtt tgtttcgaga gtcaagcgca
      481 gcctcgatct acttccaccc cattcatgca actgtgtgca tttatcaatg actcacacta
      541 gcctgtgagt gcgcgagcca ctgacgttgt cttcctatta tataacctct cttctctcgt
      601 cgtcgatttt cgcacgaagc gctctagata tccagttgaa tgtcgattgc aaaagcaaaa
      661 tgcttcgtat tgttatttcc tttcgatgtt tatcctggag aatgcaacac tgcgtcctcc
      721 gctccacaag tccgagatgc gctctctgtc actgtgagct agtgaatgga tacaagcgcg
      781 aggcgtttca atggatacat tatgtcggcc
//
