LOCUS       sequence-167813-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression vector for MS2-AID*Delta fusion protein with 
            hygromycin B selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH335_MS2-AID*Delta-Hygro
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..410
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             465..485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtcgtgacg tacggccacc atggcttcaa actttactca gttcgtgctc gtggacaatg
       61 gtgggacagg ggatgtgaca gtggctcctt ctaatttcgc taatggggtg gcagagtgga
      121 tcagctccaa ctcacggagc caggcctaca aggtgacatg cagcgtcagg cagtctagtg
      181 cccagaagag aaagtatacc atcaaggtgg aggtccccaa agtggctacc cagacagtgg
      241 gcggagtcga actgcctgtc gccgcttgga ggtcctacct gaacatggag ctcactatcc
      301 caattttcgc taccaattct gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca
      361 aagacggtaa tcctatccct tccgccatcg ccgctaactc aggtatctac agcgctggag
      421 gaggtggaag cggaggagga ggaagcggag gaggaggtag cggacctaag aaaaagagga
      481 aggtggcggc cgctggatcc atggacagcc tcttgatgaa ccggagggag tttctttacc
      541 aattcaaaaa tgtccgctgg gctaagggtc ggcgtgagac ctacctgtgc tacgtagtga
      601 agaggcgtga cagtgctaca tccttttcac tggactttgg ttatcttcgc aataagaacg
      661 gctgccacgt ggaattgctc ttcctccgct acatctcgga ctgggaccta gaccctggcc
      721 gctgctaccg cgtcacctgg ttcatctcct ggagcccctg ctacgactgt gcccgacatg
      781 tggccgactt tctgcgaggg aaccccaacc tcagtctgag gatcttcacc gcgcgcctct
      841 acttctgtga ggaccgcaag gctgagcccg aggggctgcg gcg
//
